pSB8K15FRT

BBa_K864022 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K864022

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Erik Lundin

Date created: 2012-09-23 11:00:00

Date modified: 2015-05-08 01:13:37

Low copy BioBrick temperature sensitive standard vector

Details

• Types: DnaRegion
• Roles: plasmid_vector; Plasmid_Backbone
• Sequences: BBa_K864022_sequence (Version 1)

Description

awd

Notes

The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is.

The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.

The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.

Source

Based on BBa_K864017

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K864022/1

Member of these Collections

• //plasmidbackbone/assembly
• //plasmidbackbone/copynumber/low
• //plasmidbackbone/operation
• //plasmidbackbone/version/_5
• iGEM Parts Registry
• igem_plasmid_vectors