Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: edgar andres ochoa cruz
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:40
Recombination Device composed by lox71-Cre recombinase
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K886001_sequence (Version 1) |
Description
This device was created to express the Cre recombinase enzyme and a target gene under the control of the same promoter (promoter not included in this part). The target gene needs to be amplified by PCR using primers that will insert a loxP and a lox66 sites flanking the sequence in order to integrate it in the lox71 recombination site located upstream the Cre recombinase gene.Once this device is located under the control of the desired promoter, it will express the Cre recombinase that recognizes the lox sites and inserts the target gene under the control of the same promoter.
The Cre expression levels are critical for the recombination, we created two different devices: A (Cre-Lox71) and B (Lox71-Cre) for testing if inserting the target gene either upstream or downstream the Cre gene will affect the expression efficiency of the target gene.
Notes
We used the lox71 submitted by our group, which correct the part BBa_I718017 and BBa_K537020 when compared with the published sequence at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC137435/Source
We synthesized the lox71 site and then we assembled it with the Cre recombinase part BBa_J61047| Access | Instance | Definition |
| public public | BBa_K886000 BBa_J61047 | BBa_K886000 Cre |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K886000 BBa_J61047 | 1,34 41,1077 | BBa_K886000 Cre |