Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: edgar andres ochoa cruz
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:40
Recombination Device composed by Cre-lox71
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K886003_sequence (Version 1) |
Description
This device was created to express the Cre recombinase enzyme and a target gene under the control of the same promoter (promoter not included in this part). The target gene needs to be amplified by PCR, using primers that will insert a loxP and a lox66 sites flanking the sequence, in order to integrate it in the lox71 recombination site. For this part the lox71 is located downstream the Cre recombinase gene. An alternative construction is submitted in the part BBa_K886001.Once this device is located under the control of the desired promoter, it will express the Cre recombinase that recognizes the lox sites and inserts the target gene under the control of the same promoter.
The Cre expression levels are critical for the recombination, we created two different devices: BBa_K886002 (Cre-Lox71) and BBa_K886001 (Lox71-Cre) for testing if inserting the target gene either upstream or downstream the Cre gene will affect the expression efficiency of the target gene
Notes
We used the lox71 submitted by our group BBa_K886000, which corrected the parts BBa_I718017 and BBa_K537020. Those parts are wrong when compared with the published sequence at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC137435/Source
We synthesized the lox71 site, then we assembled it downstream the Cre recombinase gene(BBa_J61047).| Access | Instance | Definition |
| public public | BBa_J61047 BBa_K886000 | Cre BBa_K886000 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J61047 BBa_K886000 | 1,1037 1046,1079 | Cre BBa_K886000 |