BBa_K891999

BBa_K891999 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K891999
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ryan Muller
Date created: 2012-10-01 11:00:00
Date modified: 2015-05-08 01:13:41

GFPT2



Types
DnaRegion

Roles
sequence_feature

DNA

Sequences BBa_K891999_sequence (Version 1)

Description

This part should be paired with GFPT1. This part codes for a 20bp sequence that is complementary to a portion of the genomic GFP coding sequence that comes after the GFPT1 binding site in E.coli Keio strains.

Notes

Blasted sequence against parent strains and identified a region with high specificity. Needs to be absent of YCCTT motifs and AAGGR.

Source

It comes from genomic GFP in E.coli Keio strains.

Sequence Annotation Location Component / Role(s)
Topo binding site
Topo binding site
Topo binding site
GFPT2 recognition probe
GFPT2 recognition probe
1,5
26,30
51,55
6,30
31,55
feature/binding non_covalent_binding_site
non_covalent_binding_site feature/binding
non_covalent_binding_site feature/binding
sequence_feature feature/misc
feature/misc sequence_feature
igem#experience
Works
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K891999/1