BBa_K892801

BBa_K892801 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K892801

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Benjamin Groves

Date created: 2012-10-02 11:00:00

Date modified: 2015-05-08 01:13:41

Repaired LovTAP (see part BBa_K191006 and BBa_K892800)

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K892801_sequence (Version 1)

Description

Part BBa_892801 is an improvement on part BBa_K191003. It fixes a frameshift deletion in LovTAP. The hybrid protein LovTAP has a light-sensitive domain (Lov: Light, oxygen, voltage) and a regulatory domain (part of the E. coli Trp repressor). The fused protein allows transmission of the conformational change induced by light (on the light-sensitive domain) to the DNA-binding domain, therefore resulting in an increase or decrease of the regulatory domain's affinity for its DNA binding site. The activation of the LovTAP protein (binding to DNA) is achieved by illumination @ 470 nm

For more details, please consult the section of LovTAP system on team EPF-Lausanne wiki, or the App page (http://2012.igem.org/Team:Washington/Optogenetics) on the University of Washington wiki.

Notes

A deletion causing a frameshift was fixed. We have also improved the annotation

Source

BBa_K191003

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K892801/1

Member of these Collections

• iGEM Parts Registry