Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Oliver Meacock
Date created: 2012-09-11 11:00:00
Date modified: 2015-05-08 01:13:45
Magnesium sensitive riboswitch (8 codon substitution)
| Types | DnaRegion |
| Roles | engineered_region Measurement |
| Sequences | BBa_K911002_sequence (Version 1) |
Description
Riboswitch that acts as a regulatory element, truncating transcripts when magnesium is not bound to the RNA. Four Mg2+ binding sites exist, giving this part significantly switch-like behavior. Binding of these sites by Mg2+ results in compaction of the regulatory region of the riboswitch, which in its unbound state acts as an anti-terminator. Loss of this anti-terminator activity results in the activity of a downstream terminator (included in this sequence) which terminates transcription of the gene. May also be affected by other divalent ions, such as manganese.Notes
Original paper mentioned 8 codon substitution of the downstream LacZ reporter gene with the first 8 codons of the MgtE gene (which is the native gene which this riboswitch controls). Our construct used LacI as the downstream regulated sequence, which requires its N-terminal domain to bind to DNA. Sequences both with and without this substitution have been submitted. This contains the substitution. Please note that to use this version effectively, an assembly method that does not leave scars (such as Gibson assembly) will be required.Source
Bacillus Genome| Sequence Annotation | Location | Component / Role(s) |
| 8 codon substitution. M-box Terminator | 487,510 95,250 284,299 | feature/protein CDS feature/misc sequence_feature feature/misc sequence_feature |