Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Nicholas
Date created: 2012-10-02 11:00:00
Date modified: 2015-05-08 01:13:45
pH Inducible Lysis System(ns)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K912033_sequence (Version 1) |
Description
This construct was developed by Northwestern's 2012 iGEM team as part of a phytase producing vector meant to self lyse in high chloride and low pH conditions. The device is designed to lyse in the presence of high chloride concentrations. The Pgad promoter in front of the lysis cassette is activated by the combination of high extracellular hydrogen ion concentration and the presence of high intracellular chloride ion concentration.Notes
None besides designing primers properlySource
Lysis:registryCP/RBS: registry
clc-ec1: PCR from genome
Pgad: PCR from vector from CUHK 2011 team
| Access | Instance | Definition |
| public public | BBa_K912029 BBa_K912012 | BBa_K912029 BBa_K912012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K912029 BBa_K912012 | 1,3044 3053,4515 | BBa_K912029 BBa_K912012 |