BBa_K914015

BBa_K914015 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K914015
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dylan Iverson
Date created: 2012-10-24 11:00:00
Date modified: 2015-05-08 01:13:46

Pbad/AraC RBS LacI[LVA(-)] Plac



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Composite

    Sequences BBa_K914015_sequence (Version 1)

    Description

    Paris Bettencourt 2012 delay system with Plac promoter. An RBS and restriction enzyme coding region could be added after the promoter to create a plasmid degradation system that is inhibited in the presence of arabinose. Removal of arabinose and dilution of LacI would trigger restriction.

    Notes

    LVA tag was removed from LacI by PCR. The delay was not functional with fast-degrading LacI.

    Source

    Registry parts.

    igem#experience
    Works
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K914015/1
     

    Attachments

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