| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K916002_sequence (Version 1)
|
Description
This a fusion protein containing TraR in its entirety fused to the N terminal fragment of GFP. This is for use with a GFP complementation experiment.
Notes
TraR was cloned into existing sites in the pMRBAD-link-NGFP plasmid (NcoI and SphI). Because it was a translational fusion, care had to be taken to ensure the protein was in frame. Also, the pMRBAD-link-CGFP plasmid was designed to use NcoI and AatII, but TraR contained two AatII sites. Cloning into the SphI site instead added two extra amino acids in the linker.
Source
TraR came from a plasmid (pCF222)provided by Dr. Clay Fuqua at University of Illinois Champaign-Urbana. The CGFP fragment came from a plasmid (pMRBAD-link-CGFP) provided by Dr. Lynne Regan at Yale University.
