| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K933001_sequence (Version 1)
|
Description
This part tests codon optimization of the theonine codons. It is prefaced by an AHL leader sequence, then a 6-codon repeat sequence of one codon, followed by an mCherry and GFP reporter. The reporters determine the amount of tRNA degradation which occurs as a result of the repeat sequence. Use to determine other codon optimization characterization.
Notes
The original RBS in this plasmid construct was discarded in favor of a weaker translation RBS for mCherry.
Source
This piece was already partially assembled by a Ph.D student working in the Richard lab at Penn State named Mike Speer. Additional work was performed to perfect the construct with an alternate RBS from the registry. Repeat sequences were ordered as ssDNA oligos.
