BBa_K950004

BBa_K950004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K950004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jakob Matthes

Date created: 2012-07-28 11:00:00

Date modified: 2015-05-08 01:13:49

firefly luciferase

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K950004_sequence (Version 1)

Description

This is a optimized firefly luciferase (C-terminal modification, see design notes).

Notes

Primers were designed to replace the C-terminal SKL aminoacids with KL.

Source

The original source is the Firefly luciferase, see http://www.ncbi.nlm.nih.gov/nuccore/AB644228.1

The modification is covered by 'One-step measurement of firefly luciferase activity in yeast' (http://www.ncbi.nlm.nih.gov/pubmed/14558144).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
luciferase	1,1650	feature/cds, CDS
XbaI	48,54	feature/misc, sequence_feature

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K950004/1

Member of these Collections

• //chassis/eukaryote/yeast
• //function/reporter/light
• iGEM Parts Registry