Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jakob Matthes
Date created: 2012-09-12 11:00:00
Date modified: 2015-05-08 01:13:49
pRS315 shuttle vector
| Types | DnaRegion |
| Roles | plasmid Plasmid |
| Sequences | BBa_K950010_sequence (Version 1) |
Description
pRS315 is a Saccharomyces/E. coli shuttle vector. The multiple cloning site has XbaI and SpeI restriction sites. EcoRI and PstI cannot be used with this plasmid. It contains the LEU2 gene for selection.Notes
EcoRI and SpeI restriction cannot be used with this vector.Source
http://genome-www.stanford.edu/vectordb/vector_descrip/PRS315.html| Sequence Annotation | Location | Component / Role(s) |
| LEU2 beta-isopropylmalate F1 origin of replication lacZ T7 promoter mcs XbaI SpeI T3 promoter PBM1 origin of replication bla beta-lactamase, ampR ampicillin | 663,1757 2698,2797 2931,3268 3091,3116 3124,3225 3148,3153 3154,3159 3243,3262 3686,3686 4444,5304 | feature/cds CDS sequence_feature feature/misc feature/cds CDS feature/promoter promoter sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/promoter promoter sequence_feature feature/misc feature/cds CDS |