Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jakob Matthes
Date created: 2012-09-12 11:00:00
Date modified: 2015-05-08 01:13:49
pRS316 shuttle vector
| Types | DnaRegion |
| Roles | plasmid Plasmid |
| Sequences | BBa_K950011_sequence (Version 1) |
Description
pRS316 is a Saccharomyces/E. coli shuttle vector. The multiple cloning site has XbaI and SpeI restriction sites. EcoRI and PstI cannot be used with this plasmid. It contains the URA3 gene for selection.Notes
EcoRI and SpeI restriction cannot be used with this vector.Source
http://genome-www.stanford.edu/vectordb/vector_descrip/COMPLETE/PRS316.SEQ.html| Sequence Annotation | Location | Component / Role(s) |
| URA3 orotidine-5'-phosphate lacZ' beta-galactosidase alpha peptide T7 promoter mcs XbaI SpeI T3 promoter bla beta-lactamase, ampR ampicillin resistance centromere VI ARSH4 autonomous replicating sequence histone 4 F1 origin of replication PMB1 origin of replication | 411,1214 1800,2137 1966,1985 1993,2100 2017,2022 2023,2028 2112,2131 3313,4173 4315,4431 4432,4806 1567,1666 2555,2555 | feature/cds CDS feature/cds CDS promoter feature/promoter feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc promoter feature/promoter feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc |