Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hongmei Du
Date created: 2013-06-20 11:00:00
Date modified: 2015-05-08 01:13:51
UV- Responsive Wintergreen Odor
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K994000_sequence (Version 1) |
Description
The UV-responsive wintergreen odor generator catalyzes the conversion of the precursor salicylic acid to the wintergreen odor methyl salicylate under extensive UV irradiation. The biosynthetic device is composed of two transcriptional devices: Rec A (SOS) promoter (BBa_J22106) and a wintergreen odor enzyme generator (BBa_J45119). The odor generator takes as input extensive UV irradiation and produce as output an enzyme that catalyzes production of an odor from a chemical precursor.Notes
The primers for DNA sequncingDu-5F：5???-AGTGGAGTGCCTGGTTCAT -3???；626bp～644bp
Du-3R：5???-AAATCTATTTCATATTGCTTG-3???；799bp～819bp
Source
Kit Plates| Sequence Annotation | Location | Component / Role(s) |
| BBa_J22106 BBa_B0032 RBS-3\Weak RBS CDS Start BBa_J45004 Stop BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_J45119 | 1,192 201,213 201,213 207,210 220,1293 220,222 220,1293 1290,1293 1302,1381 1313,1356 1390,1430 1397,1416 1417,1430 1423,1423 201,1430 | feature/BioBrick engineered_region feature/BioBrick engineered_region feature/rbs ribosome_entry_site feature/rbs ribosome_entry_site feature/cds CDS feature/misc sequence_feature engineered_region feature/BioBrick stop_codon feature/stop feature/BioBrick engineered_region feature/stem_loop stem_loop feature/BioBrick engineered_region feature/stem_loop stem_loop polyA_site feature/polya stop_codon feature/stop engineered_region feature/BioBrick |