BBa_M10020

BBa_M10020 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M10020
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nikhil Sharma
Date created: 2009-05-09 11:00:00
Date modified: 2015-05-08 01:13:51

{˂
TraT˃
} C-terminal Display Protein



    • Details

    Types
    DnaRegion

    Roles
    Other

    sequence_feature

    Sequences BBa_M10020_sequence (Version 1)

    Description

    later

    Notes

    -Had to remove the prepro sequence

    Source

    Construction of {} part

    PCR Ons001F/Ons002R on TraT (700 bp, BamHI/EcoRI)
    Digest pBca9495CA-Bca1144#5 (BamHI/EcoRI, 3039+910, L)
    Product is pBca9495CA-M10020 {}

    Ons001F Forward oligo for TraT
    ccataGAATTCatgAGATCTtgtggtgcgatgagcacagc
    Ons002R Reverse oligo for TraT
    ctgatGGATCCgagaatatttgcgattgatttgg

    igem#experience
    None
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_M10020/1
     

    Attachments

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