BBa_M12100

BBa_M12100 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M12100
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2010-11-24 12:00:00
Date modified: 2015-05-08 01:13:55

PLambda : B0034 : RFP in BioScaffold Form, a (0,5
7,2] BioScaffold part



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_M12100_sequence (Version 1)

Description

This is a test part for validation of a BioScaffold project. The part is a red fluorescent protein constitutively expressed by the PLambda promoter and the strong Elowitz ribosome binding site.

Notes

See documentation about BioScaffold restriction sites- specifically FalI and PsrI- for more information about the requirements for BioScaffold designs.

Source

This is a modified version of the BBa_I763007 part and contains a truncated terminator primarily for ease of use with BioScaffold-oriented priming and assembly.

Sequence Annotation Location Component / Role(s)
PsrI Site
Left BioScaffold Padding
Right BioScaffold Padding
PsrI Site
Lambda Promoter
Elowitz RBS
RFP
Truncated Terminator
7,19
1,19
879,896
879,891
20,68
77,88
95,800
809,896
non_covalent_binding_site feature/binding
feature/BioBrick engineered_region
engineered_region feature/BioBrick
feature/binding non_covalent_binding_site
promoter feature/promoter
feature/rbs ribosome_entry_site
CDS feature/cds
feature/misc sequence_feature
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_M12100/1