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BBa_M1529

BBa_M1529 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_M1529

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sarah Allred

Date created: 2012-04-16 11:00:00

Date modified: 2015-05-08 01:13:56

Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag with secretion tag and GFP

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Details

Types	DnaRegion
Roles	Composite engineered_region
Sequences	BBa_M1529_sequence (Version 1)

Description

This is a thermal hysteresis (antifreeze) protein with an 8-His tag for protein purification. It has up to 100 times the specific activity of fish antifreeze proteins. There are four variants of the protein (YL1-4). This is variant YL4. There is a secretion tag, and a portion of the sequence causes the cell to secrete GFP as well to measure the activity of the promoter.

Notes

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Source

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Components

Access	Instance	Definition
public public public public public public public public public	BBa_K208010 BBa_K208006 BBa_M1524 BBa_M1528 BBa_B0034 BBa_K208006 BBa_K208000 BBa_M1528 BBa_B0015	BBa_K208010 BBa_K208006 BBa_M1524 BBa_M1528 BBa_B0034 BBa_K208006 BBa_K208000 BBa_M1528 BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K208010 BBa_K208006 BBa_M1524 BBa_M1528 BBa_B0034 BBa_K208006 BBa_K208000 BBa_M1528 BBa_B0015	1,220 229,414 421,927 936,4544 4553,4564 4573,4758 4765,5484 5493,9101 9110,9238	BBa_K208010 BBa_K208006 BBa_M1524 BBa_M1528 BBa_B0034 BBa_K208006 BBa_K208000 BBa_M1528 BBa_B0015