BBa_M31107

BBa_M31107 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M31107
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Lee
Date created: 2008-04-30 11:00:00
Date modified: 2015-05-08 01:13:59

Produces Beta-carotene from the biochemical input GGPP



Types
DnaRegion

Roles
Composite

engineered_region

Description

This part contains 3 genes, each of which codes for an enzyme in the carotenoid synthesis pathway:
CrtB-Phytoene synthase
CrtI-Phytoene desaturase
CrtY-Lycopene cyclase
(CrtB) (CrtI) (CrtY)
The conversion process is GGPP->Phytoene->Lycopene->B-carotene

Order:
1) Constitutive promoter BBa_J23100
2) CrtY
3) RBS BBa_B0034
4) CrtI
5) RBS BBa_J23100
6) CrtB
7) Terminator B0015

Notes

Contact biomagik@mit.edu for specific questions.

Source

Each gene is taken from a different bacterial organism:
CrtB-R capsulatus
CrtI-S solfataricus
CrtY-C pelagibacter

Our sequence has been codon optimized for the Lactobacillus acidophilus strain NCFM.

The promoter, RBS and terminator were taken from the parts.mit.edu website.

igem#sampleStatus
No part sequence
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_M31107/1