Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Lee
Date created: 2008-04-30 11:00:00
Date modified: 2015-05-08 01:13:59
Produces Beta-carotene from the biochemical input GGPP
| Types | DnaRegion |
| Roles | Composite engineered_region |
Description
This part contains 3 genes, each of which codes for an enzyme in the carotenoid synthesis pathway:CrtB-Phytoene synthase
CrtI-Phytoene desaturase
CrtY-Lycopene cyclase
(CrtB) (CrtI) (CrtY)
The conversion process is GGPP->Phytoene->Lycopene->B-carotene
Order:
1) Constitutive promoter BBa_J23100
2) CrtY
3) RBS BBa_B0034
4) CrtI
5) RBS BBa_J23100
6) CrtB
7) Terminator B0015
Notes
Contact biomagik@mit.edu for specific questions.Source
Each gene is taken from a different bacterial organism:CrtB-R capsulatus
CrtI-S solfataricus
CrtY-C pelagibacter
Our sequence has been codon optimized for the Lactobacillus acidophilus strain NCFM.
The promoter, RBS and terminator were taken from the parts.mit.edu website.