Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kate Broadbent
Date created: 2008-05-12 11:00:00
Date modified: 2015-05-08 01:13:59
Calcium channel-apoaequorin fusion
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_M31234_sequence (Version 1) |
Description
This part encodes for the homo sapiens neuronal voltage-gated calcium ion channel gamma-3 subunit linked to Aequorea victoria apoaequorin. The ion channel is expressed in the membrane of the presynaptic cleft, with an apoaequorin linked to the C-terminal of the channel. If coelenterazine is present within the neuron, it binds to the apoaequorin along with oxygen. When an action potential reaches the modified ion channel, calcium ions flood in to the cell, and 2 or 3 of them bind to the apoaequorin. When the calcium ions bind, the coelenterazine is oxidized, and light in the form of bioluminescence is emitted as a byproduct. This part, when used in tandem with coelenterazine will show the activity of neurons by bioluminescing whenever an action potential reaches the synapse.Notes
Designing an appropriate linkerSource
Accession number for Homo sapiens neuronal voltage gated calcium channel gamma-3 subunit = AF100346Accession number for Aequorea victoria apoaequorin = E14214
| Sequence Annotation | Location | Component / Role(s) |
| calcium channel linker apoaequorin stop | 1,3 946,984 985,988 1843,1845 | feature/start start_codon feature/cds CDS start_codon feature/start stop_codon feature/stop |