BBa_M33336

BBa_M33336 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M33336

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Christopher Brunson

Date created: 2010-06-04 11:00:00

Date modified: 2015-05-08 01:14:01

Inorganic Mercury-Inducible Promoter repressed by merR

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_M33336_sequence (Version 1)

Description

This operator/promoter sequence regulates the mer operon in Thermus thermophilus HB 27; in the absence of inorganic mercury, merR, the repressor protein (BBa_M33335) binds to this sequence, preventing transcription of the downstream operon. In the presence of inorganic mercury, merR undergoes a conformation change, unblocking this promoter and allowing the downstream operon to be transcribed.

Notes

We only knew that the promoter sequence was located 69 bp upstream of the merR operon, so we isolated the 160 bp sequence directly upstream of the operon.

Source

Thermus thermophilus HB27, complete genome
GenBank: AE017221.1
774845-77505

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M33336/1

Member of these Collections

• iGEM Parts Registry