Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rahul Sastry, Anjan Katta
Date created: 2013-06-09 11:00:00
Date modified: 2015-05-08 01:14:02
fnr (fumarate and nitrate reductase) coding sequence
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_M36066_sequence (Version 1) |
Description
This part codes for the fnr protein, a transcriptional activator in E. coli that is sensitive to oxygen levels. The protein is has an iron-sulfur code that, when bound to oxygen, undergoes a conformational change that reduces its ability to activate transcription. This particular coding sequence includes a nuclear localization sequence at the N-terminus (for application in eukaryotes) and a stop codon.Notes
NLS sequences were inserted at the N terminus of the sequence in order to minimize negative effects on the functionality of the protein.Source
The 753-bp FNR coding sequence was isolated from K-12 DH10B E. coli, as reported on the E. coli Wiki and the Generic Genome Browser (http://ecoliwiki.net/colipedia/index.php/fnr:Gene). The coding sequence for the NLS was obtained from Clontech???s pGAD424 plasmid, which is used to carry out 2 hybrid assays.| Sequence Annotation | Location | Component / Role(s) |
| fnr coding sequence stop | 1,747 748,750 | feature/dna sequence_feature stop_codon feature/stop |