Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dan Sakaguchi
Date created: 2013-10-23 11:00:00
Date modified: 2015-05-08 01:14:02
Wild-type miraculin protein with His-tag at C-terminus
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_M36080_sequence (Version 1) |
Description
This is the coding gene for the protein naturally found in the miracle berry (Synsepalum dulcificum). The protein is his-tagged at the C-terminus (with a poly-glycine linker) for purification. In the berry the protein is produced as a glycoprotein dimer, in which form it has taste-modifying properties.Notes
Miraculin naturally has exposed histidine tags which allow for metal ion purification, yet attaching the his-tag should allow for greater concentrations of purified protein.Source
The sequence is from the UniProt database (P13087) without the C-terminus signal peptide.| Sequence Annotation | Location | Component / Role(s) |
| Miraculin Protein Poly-glycine linker 6xHis tag | 1,573 574,582 583,600 | feature/protein CDS feature/misc sequence_feature tag feature/tag |