Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joseph Shih
Date created: 2013-04-22 11:00:00
Date modified: 2015-05-08 01:14:03
Mammalian actuator test rig
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_M36096_sequence (Version 1) |
Description
Mammalian test vector for actuators. Has a CMV promoter for constitutive expression in mammalian cells. Comes with high copy number origin and ampicillin resistance; can replace with low copy number origin and kanamycin resistance if ordering from DNA 2.0.Notes
Developed by DNA2.0 from pcDNA3.1; IP free.Source
pJexpress609 on the DNA2.0 website.| Sequence Annotation | Location | Component / Role(s) |
| rpo_bla transcription terminator CMV promoter student actuators go here BGH polyA SV40 promoter puromycinR SV40 polyA AmpR Pamp pUCori | 1,425 507,1108 1119,1188 1195,1320 1432,1775 1886,2485 2613,2743 2789,3649 3650,3827 3828,4632 | feature/stop stop_codon promoter feature/promoter sequence_feature feature/misc feature/polya polyA_site promoter feature/promoter feature/cds CDS feature/polya polyA_site CDS feature/cds feature/promoter promoter sequence_feature feature/misc |