BBa_M36160

BBa_M36160 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M36160

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Eric Kofman, Matt Kandath

Date created: 2012-06-03 11:00:00

Date modified: 2015-05-08 01:14:03

T7 17.5 protein (holin)

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_M36160_sequence (Version 1)

Description

Coding sequence for 17.5 protein in T7 phage. The 17.5 protein functions as a holin: in gram-negative bacteria, the holin degrades the inner peptidoglycan cell wall. For complete lysis to occur, an endolysin protein is also required (in T7 phage, this is the 3.5 protein).

This sequence should be preceded by a promoter.

Notes

Note: this sequence begins with a GTG start codon when expressed naturally; in our design, we opted to change the start codon to ATG to optimize for E. coli

Source

http://www.ncbi.nlm.nih.gov/nuccore/NC_001604.1?report=genbank&from=36344&to=36547

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M36160/1

Member of these Collections

• iGEM Parts Registry