Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Victoria Robles, Kyung Ri Park, Ken Xiong
Date created: 2011-12-09 12:00:00
Date modified: 2015-05-08 01:14:04
Copper Sensor --> PoPS
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_M36279_sequence (Version 1) |
Description
This device sends PoPs signals in response to the presence of copper. It consists of a strong constitutive promoter, a bicistronic RBS, copZ, another bicistronic RBS, copY, a transcription terminator, and cop promoter. The copY protein represses the cop promoter, but if copper attaches to copY, copY will release from the promoter, and PoPs will be produced. The copZ protein acts as a copper chaperone, transporting copper to copY attached to the cop promoter.Notes
Since we were testing our device in E. coli, we wanted to use copper regulating proteins not already found in E. coli. The regular cop operon consists of more proteins, however, we chose just copY and copZ because these were the only functions we were interested in giving our device.Source
copY, copZ, and the cop promoter are all parts from the copper-regulating cop operon found in the oral bacterium Streptococcus mutans JH1005.| Access | Instance | Definition |
| public public public public public public public | BBa_M36287 BBa_M36288 BBa_M36286 BBa_M36289 BBa_M36285 BBa_M36291 BBa_M36290 | BBa_M36287 BBa_M36288 BBa_M36286 BBa_M36289 BBa_M36285 BBa_M36291 BBa_M36290 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M36287 BBa_M36288 BBa_M36286 BBa_M36289 BBa_M36285 BBa_M36291 BBa_M36290 | 1,36 45,132 139,342 351,438 445,786 795,876 885,941 | BBa_M36287 BBa_M36288 BBa_M36286 BBa_M36289 BBa_M36285 BBa_M36291 BBa_M36290 |