Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Federico C. Rodriguez
Date created: 2014-04-16 11:00:00
Date modified: 2015-05-08 01:14:08
Uranium Inducible (urcA) Promoter
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_M45113_sequence (Version 1) |
Description
The promoter was designated urcA for uranium response in the bacterium Caulobacter. It is activated in the presence of the uranyl cation, a soluble form of uranium (Hillson et al., 2007).The uranyl ion (UO2,2+ ) is the most water-soluble and bioavailable form of uranium and poses the greatest threat to human health. Due to the ease of uranyl ion spread through groundwater systems, most bioremediation strategies attempt to prevent contaminating uranium spread by utilizing microorganisms to reduce the oxidation state of uranium from U(VI), found in uranyl, to less soluble forms of uranium, including U(IV)(Hillson et al., 2007).
The urcA promoter is specific for uranium and has little cross specificity for nitrate (<400 M), lead (<150 M), cadmium (<48 M), or chromium (<41.6 M). It was induced 27.5-fold under uranium stress, but was not upregulated in response to other heavy metals in the test screen. For this reason, the urcA promoter was selected as a candidate to drive uranium reporter constructs(Hillson et al., 2007).
Usually promoters from Caulobacter species will include at least one uranium-specific m_5 motif sequence. The urcA promoter contains two matches to this motif, located 107 and 55 bp upstream of the putative +1 site (Hillson et al., 2007).
Reference:
Nathan J. Hillson, Ping Hu, Gary L. Andersen and Lucy Shapiro. Caulobacter crescentus as a Whole-Cell Uranium Biosensor. Appl. Environ. Microbiol. 2007, 73(23):7615. DOI: 10.1128/AEM.01566-07.
This reference was retrieved from: file:///C:/Users/Owner/Desktop/Appl.%20Environ.%20Microbiol.-2007-Hillson-7615-21.pdf
Notes
The sequence complied with RFC-10 standard assembly, so no design considerations had to be considered during the creation of the sequence as a biobrick.Source
This part comes from Caulobacter crescentus CB15 section 318 of 359 of the completegenome, subsection 7998-8971, which may be found in Genbank at accession number AE005992.1 (Hillson et al., US Patent, Feb. 6 2008).
Reference:
Nathan J. Hillson, Ping Hu, Gary L. Andersen and Lucy Shapiro. Heavy Metal Biosensor. US Patent Application Publication. US20110117590. Feb. 6 2008.
This reference was retrieved from: file:///C:/Users/Owner/Desktop/US20110117590.pdf
| Sequence Annotation | Location | Component / Role(s) |
| Promoter Uranium-inducible m_5 motif 2 Uranium-inducible m_5 motif 1 | 1,974 877,903 825,851 | promoter feature/promoter sequence_feature feature/misc feature/misc sequence_feature |