Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nicolas Quach
Date created: 2016-10-23 11:00:00
Date modified: 2016-12-09 07:58:12
Streptavidin binding outer membrane protein
| Types | DnaRegion |
| Roles | Protein_Domain polypeptide_domain |
| Sequences | BBa_M50000_sequence (Version 1) |
Description
Cell surface display protein with N-terminal and C-terminal streptavidin binding domain. eCPX-nSA-cNano will be used to make E. coli bind to streptavidin coated magnetic beads, enabling bacterial capture.Notes
eCPX was developed as a biterminal display scaffold, and thus both N and C termini are available for fusion with other domains. An N terminal fusion with a streptavidin binding domain had already been created by Rice and Daugherty for their paper on eCPX; we wished to increase the affinity by fusing another (stronger binding) streptavidin binding domain to the C terminusSource
Derived from E. coli outer membrane protein OmpX. eCPX is an engineered version of OmpX developed by Rice and Daugherty (Protein Eng. Des. Sel., 2008, pg 435-442)| Sequence Annotation | Location | Component / Role(s) |
| NanoTag SA-binding domain Ser-Gly linker | 635,679 86,130 617,634 | feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site sequence_feature feature/misc |