BBa_M50023

BBa_M50023 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M50023
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Elisa Liu
Date created: 2016-10-26 11:00:00
Date modified: 2016-10-27 06:56:05

UV sensitive promoter



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_M50023_sequence (Version 1)

Description

The vector will be expressed in an E. coli host. We've included the BCA1(FW) cut site and BCA1 (Rev) site on either side of our UV (360 nm) sensitive promoter. We want to use this promoter to drive the expression of GFP in the plasmid EColi_Promoter_Cassette.

Notes

We wanted to use a promoter that is compatible with E. Coli. In addition, we have flanked the promoter with BSA1 sequences that will allow the BSA enzyme to cut the promoter into another plasmic.

Source

We are using the same promoter as the iGEM Columbian Team 2007.

Sequence Annotation Location Component / Role(s)
Bsa1-fw
Bsa1_rev
1,11
87,98
feature/binding non_covalent_binding_site
non_covalent_binding_site feature/binding
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_M50023/1