Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lucy Maynard, Doris Mai and Amy Weissenbach
Date created: 2016-12-11 12:00:00
Date modified: 2016-12-12 12:59:47
PETase double mutant I208V and R90A
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_M50054_sequence (Version 1) |
Description
This is a mutant of PETase that combines two mutations designed by the 2016 iGEM Tianjin team. Wild type PETase shows emzyme ability to hydrolyze Polyethylene terephthalate (PET), commonly used for plastics. The two mutations are the 208th amino acid (changed from isoleucine(I) to valine(V)) and the 90th amino acid (changed from arginine (R) to alanine (A)), and each individual mutant has been showed by the Tianjin team to improve the catalytic activity of PET. This sequence combines both mutations, and is optimized for Escherichia coli.Notes
The design is based on the hypothesis that the combination of the two mutations will further enhance the PETase catalytic activity.Source
Wild type PETase sequence is originally from Ideonella sakaiensis.| Sequence Annotation | Location | Component / Role(s) |
| start codon PETase coding region 6 hig tag stop codon I208V R90A | 1,3 4,873 874,891 892,894 622,624 268,270 | start_codon feature/start feature/dna sequence_feature tag feature/tag stop_codon feature/stop feature/mutation sequence_alteration sequence_alteration feature/mutation |