gmR

BBa_P1014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_P1014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Tom Knight

Date created: 2006-06-10 11:00:00

Date modified: 2015-05-08 01:14:12

gentamicin resistance cassette aph(2)

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_P1014_sequence (Version 1)

Description

Novel gentamicin resistance gene. Derived from Genbank DQ208936, pTEX5500ts, kind gift of Barbara E. Murray. This, in turn was derived from Genbank AF016483. See pmid=16391062, pmid=9593155

Notes

XbaI site at 660 removed by mutating c->t which also improves codon usage.
gatgatttttacagggatattttagagaacgaaatctac,P1014-XF
gtagatttcgttctctaaaatatccctgtaaaaatcatc,P1014-XR

Source

DQ208936

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
aph(2)	120,1025	CDS, feature/cds
stem_loop	1040,1070	stem_loop, feature/stem_loop

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_P1014/1

Member of these Collections

• //chassis/prokaryote/mflorum
• //direction/forward
• //plasmidbackbone/component/selectionmarker/antibioticresistance
• iGEM Parts Registry