lacITst

BBa_S03469 Version 1

Component

Source: http://parts.igem.org/Part:BBa_S03469

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: ETH Zurich iGEM Team 2005

Date created: 2006-05-01 11:00:00

Date modified: 2015-05-08 01:14:23

Lambda-lacI input system TESTER (IPTG controlled lacI+pL efficiency)

Details

• Types: DnaRegion
• Roles: Measurement; engineered_region
• Sequences: BBa_S03469_sequence (Version 1)

Description

Constitutive (tetR) lacI-production (lacI+LVA) to block promoter (lacI+pL) in ground state of a 2-state system. lacI+pL can be activated, e.g. over IPTG, thus leading to the production of CFP (yellow fluorescence). This device allows to estimate repressed (lacI) activity/full activity under ITPG absence/presence.

Notes

For more details see Sensing Device and Event Processing Device in the ETH Team iGEM2005 WiKi documentation.

Source

Registry 7.05

Components

Access	Instance	Definition
public	BBa_S03334	input-lacI
public	BBa_E0422	BBa_E0422

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_S03334	1,1433	input-lacI
BBa_E0422	1442,2358	BBa_E0422

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_S03469/1

Member of these Collections

• iGEM Parts Registry