7x His

BBa_T2001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_T2001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reshma Shetty
Date created: 2009-08-10 11:00:00
Date modified: 2015-05-08 01:14:52

7x His tag head domain



Types
DnaRegion

Roles
polypeptide_domain

Protein_Domain

Sequences BBa_T2001_sequence (Version 1)

Description

This is a 6x His tag head domain designed to encode the N-terminus of a protein. His tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.

Notes

Both Histidine codons were used to minimize repeats. An ATG start codon was used.

Source

This is a synthetic sequence.

Sequence Annotation Location Component / Role(s)
start codon
7x his tag
preferred AAA +2 codon
1,3
7,27
4,6
start_codon feature/start
feature/tag tag
feature/start start_codon
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_T2001/1