Strep-tag

BBa_T2013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_T2013
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reshma Shetty
Date created: 2009-08-10 11:00:00
Date modified: 2015-05-08 01:14:52

Strep-tag II head domain (WSHPQFEK)



Types
DnaRegion

Roles
polypeptide_domain

Protein_Domain

Sequences BBa_T2013_sequence (Version 1)

Description

This is a Strep tag head domain designed to be at the N-terminus of a protein. Strep tags are typically used for detection, purification and immobilization of proteins.

Notes

This domain encodes the Strep tag sequence WRHPQFGG. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like E. coli, humans, mouse, B. subtilis, C. elegans, Streptomyces coelicolor and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.

Source

This is a synthetic sequence.

Sequence Annotation Location Component / Role(s)
start codon
preferred AAA +2 codon
strep tag II
1,3
4,6
7,30
start_codon feature/start
feature/start start_codon
tag feature/tag
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_T2013/1