BBa_K209083
BBa_K209083 Version 1 (Component)
PAKA-N-linker-NFA

pNFAT
BBa_K838003 Version 1 (Component)
NFAT response element

BBa_K948001
BBa_K948001 Version 1 (Component)
ANF promoter and eGFP reporter

BBa_K1103000
BBa_K1103000 Version 1 (Component)
NFκB Binding Element

BBa_K1103001
BBa_K1103001 Version 1 (Component)
NF-κB Binding Element

BBa_K1103004
BBa_K1103004 Version 1 (Component)
NFκB-IκB-GFP

ZEC
BBa_K1018001 Version 1 (Component)
she ble gene conferring Zeocin resistance

BBa_K1325001
BBa_K1325001 Version 1 (Component)
ranscriptionfactor

Bacillus subtilis Collection
bsu_collection Version 1 (Collection)
This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.

BBa_K323025
BBa_K323025 Version 1 (Component)
ATG cCFP link ZNF HIVC His stop

BBa_K323029
BBa_K323029 Version 1 (Component)
CMV ATG cCFP link ZNF HIVC His stop - part of mammalian split/FRET system

BBa_K323042
BBa_K323042 Version 1 (Component)
cYFP link ZNF HIVC under T7 promoter

BBa_K323069
BBa_K323069 Version 1 (Component)
cCFP link ZNF HIVC under T7 promoter

BBa_K323071
BBa_K323071 Version 1 (Component)
ATG cCFP link ZNF HIVC

BBa_K917004
BBa_K917004 Version 1 (Component)
nfsI nitroreductase gene from Enterobacter cloacae

BBa_K1325003
BBa_K1325003 Version 1 (Component)
invF??????ranscriptionfactor

BBa_K1456015
BBa_K1456015 Version 1 (Component)
NF-κB (Nuclear Factor kappa B) ROS response element

BBa_K1362470
BBa_K1362470 Version 1 (Component)
sfGFP_T65C sequence (Mutated <partinfo>BBa_I746917</partinfo>)

GreenFET1
BBa_K1586000 Version 1 (Component)
Synthetic toehold riboswitch - J23100

GreenFET1T
BBa_K1586001 Version 1 (Component)
Synthetic toehold riboswitch - T7

BBa_K1712001
BBa_K1712001 Version 1 (Component)
Haemophilus influenzae FABI

BBa_K2036003
BBa_K2036003 Version 1 (Component)
SnRK2.2,SNF1-related protein kinase 2.2

BBa_K1915000
BBa_K1915000 Version 1 (Component)
LTNF10

BBa_K1915001
BBa_K1915001 Version 1 (Component)
Inducible Lac promoter+LTNF10

BBa_K1915998
BBa_K1915998 Version 1 (Component)
LTNF Scar 2

BBa_K1915999
BBa_K1915999 Version 1 (Component)
LTNF Scar1

BBa_K2148012
BBa_K2148012 Version 1 (Component)
iRFP (near-infrared) C.reinhardtii-CO

BBa_K2074132
BBa_K2074132 Version 1 (Component)
Safety Tag Information

BBa_K1915002
BBa_K1915002 Version 1 (Component)
LTNF15

BBa_K1915003
BBa_K1915003 Version 1 (Component)
Inducible Lac promoter+LTNF15

BBa_K2002001
BBa_K2002001 Version 1 (Component)
Infared Fluorescent Protein

BBa_K2020046
BBa_K2020046 Version 1 (Component)
CNF-Synthetase for use in E.coli

BBa_M50056
BBa_M50056 Version 1 (Component)
PETase double mutant I208V and R90A full plasmid information

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K209013
BBa_K209013 Version 1 (Component)
Aar1 C-D part, NFA (aa1-359) (Dictyostelium discoideum)

BBa_K203119
BBa_K203119 Version 1 (Component)
NfKB Responsive promoter

IFP
BBa_K256008 Version 1 (Component)
Infrared Protein (IFP 1.4)

BBa_K187138
BBa_K187138 Version 1 (Component)
infA ORF forward primer

BBa_K187139
BBa_K187139 Version 1 (Component)
infA ORF reverse primer

BBa_K187140
BBa_K187140 Version 1 (Component)
infC ORF forward primer

BBa_K187141
BBa_K187141 Version 1 (Component)
infC ORF reverse primer

BBa_K256033
BBa_K256033 Version 1 (Component)
Infrared signal reporter (J23119:IFP:J23119:HO1)