BBa_K615004
BBa_K615004 Version 1 (Component)
Zif268 binding site-His3-URA3 construct for one-hybrid selection

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

BBa_K113021
BBa_K113021 Version 1 (Component)
Total construct: T7 polymerase + GFP/RFP + repressors + overlapping T7 promoter

BBa_K143077
BBa_K143077 Version 1 (Component)
P43-gsiB GFP expression construct

BBa_K143078
BBa_K143078 Version 1 (Component)
P43-spoVG GFP expression construct

BBa_K143079
BBa_K143079 Version 1 (Component)
Pveg-spoVG GFP expression construct

BBa_K143080
BBa_K143080 Version 1 (Component)
P43-gsiB RFP expression construct

BBa_K143081
BBa_K143081 Version 1 (Component)
P43-spoVG RFP expression construct

BBa_K143082
BBa_K143082 Version 1 (Component)
Pveg-spoVG RFP expression construct

BBa_K082000
BBa_K082000 Version 1 (Component)
reconstruct strong RBS

Regional
BBa_K106676 Version 1 (Component)
Regional silencing construct

BBa_K112410
BBa_K112410 Version 1 (Component)
cadC promoter and GFP reporter construct

BBa_K116646
BBa_K116646 Version 1 (Component)
Intermediate part to construct a synchronizer

BBa_K116648
BBa_K116648 Version 1 (Component)
Intermediate part to construct a synchronizer

BBa_K152010
BBa_K152010 Version 1 (Component)
TB1 RNAi construct

BBa_K152011
BBa_K152011 Version 1 (Component)
GFP RNAi construct

BBa_K112409
BBa_K112409 Version 1 (Component)
RecA reporter GFP construct

BBa_K112411
BBa_K112411 Version 1 (Component)
cadAB promoter GFP reporter construct

BBa_K112412
BBa_K112412 Version 1 (Component)
grpE promoter GFP reporter construct

BBa_K112413
BBa_K112413 Version 1 (Component)
ptet promoter GFP reporter construct

BBa_K112414
BBa_K112414 Version 1 (Component)
spv promoter and GFP reporter construct

BBa_K093014
BBa_K093014 Version 1 (Component)
Nuclease construct

BBa_J70102
BBa_J70102 Version 1 (Component)
Left 6 his test part for library construction part.

BBa_J70104
BBa_J70104 Version 1 (Component)
Right 6 his test part for library construction part.

BBa_J69604
BBa_J69604 Version 1 (Component)
lacI Inverter Test Construct A

BBa_J69616
BBa_J69616 Version 1 (Component)
tetR Inverter Test Construct B

BBa_P111101
BBa_P111101 Version 1 (Component)
SAMT Whole construct

BBa_P121101
BBa_P121101 Version 1 (Component)
SAMT whole construct (F2620)

BBa_P111114
BBa_P111114 Version 1 (Component)
LMS complete construct (R0040, B0034 optimized)

BBa_P121114
BBa_P121114 Version 1 (Component)
LMS complete construct (F2620, B0034 optimized)

BBa_P111115
BBa_P111115 Version 1 (Component)
LIS complete construct (R0040, B0034 optimized)

BBa_P121115
BBa_P121115 Version 1 (Component)
LIS complete construct (F2620, B0034 optimized)

BBa_P111116
BBa_P111116 Version 1 (Component)
Oe23 complete construct (R0040, B0034 optimized)

BBa_P121116
BBa_P121116 Version 1 (Component)
Oe23 complete construct (F2620, B0034 optimized)

BBa_P111117
BBa_P111117 Version 1 (Component)
Myrcene Synthase complete construct (R0040, B0034 optimized)

BBa_P121117
BBa_P121117 Version 1 (Component)
Myrcene Synthase complete construct (F2620, B0034 optimized)

BBa_K091223
BBa_K091223 Version 1 (Component)
AND Gate supporting construct

BBa_K284080
BBa_K284080 Version 1 (Component)
Upstream construction flanking FSTK gene

BBa_K284090
BBa_K284090 Version 1 (Component)
Downstream construction flanking FSTK gene

BBa_K241000
BBa_K241000 Version 1 (Component)
Nuclease construct, no sense promoter.

BBa_K233325
BBa_K233325 Version 1 (Component)
The complete turing machine construct

BBa_K331018
BBa_K331018 Version 1 (Component)
Lumazine Synthase Microcompartment Test Construct (ECFP & EYFP with N-Terminal Arginine Tag)

BBa_K331019
BBa_K331019 Version 1 (Component)
Lumazine Synthase Microcompartment Test Construct (ECFP & EYFP with C-Terminal Arginine Tag)

BBa_J72042
BBa_J72042 Version 1 (Component)
pMLLCK 2AB Construction Plasmid

BBa_J72043
BBa_J72043 Version 1 (Component)
pMLLKA 2AB Construction Plasmid

BBa_J72044
BBa_J72044 Version 1 (Component)
pMLLAC 2AB Construction Plasmid

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.