BBa_K1390019BBa_K1390019 Version 1 (Component)Complete sMMO of Methylococcus capsulatus
BBa_K801090BBa_K801090 Version 1 (Component)phenylalanine ammonia lyase (PAL) + yeast consensus sequence
BBa_K801091BBa_K801091 Version 1 (Component)phenylalanine ammonia lyase (PAL) coding region
BBa_K415502BBa_K415502 Version 1 (Component)SMAD5 L1L2 MammoBlock Entry Vector
BBa_K415503BBa_K415503 Version 1 (Component)EYFP_2a_rtta3_ff5 L1L2 MammoBlock Entry Vector
CMVBBa_K415514 Version 1 (Component)pCMV L4R1 MammoBlock Entry Vector
BBa_K415513BBa_K415513 Version 1 (Component)pWRE_SV40 L4R1 MammoBlock Entry Vector
CFOSBBa_K415512 Version 1 (Component)pCFOS L4R1 MammoBlock Entry Vector
BBa_K415511BBa_K415511 Version 1 (Component)pPAI2 L4R1 MammoBlock Entry Vector
BBa_K415508BBa_K415508 Version 1 (Component)phEF1a L4R1 MammoBlock Entry Vector
EGSHBBa_K415507 Version 1 (Component)pEGSH L4R1 MammoBlock Entry Vector
BBa_K415505BBa_K415505 Version 1 (Component)VgEcR_2A_RxR L1L2 MammoBlock Entry Vector
BBa_K415504BBa_K415504 Version 1 (Component)Rtta3_2a_Hygro L1L2 MammoBlock Entry Vector
BBa_M36715BBa_M36715 Version 1 (Component)mmoX (methane monooxygenase subunit A, alpha chain)
BBa_M36718BBa_M36718 Version 1 (Component)mmoY (methane monooxygenase subunit A, beta chain)
BBa_M36720BBa_M36720 Version 1 (Component)mmoB (methane monooxygenase subunit B)
BBa_M36721BBa_M36721 Version 1 (Component)mmoZ (methane monooxygenase subunit A, gamma chain)
BBa_M36722BBa_M36722 Version 1 (Component)mmoD (methane monooxygenase subunit D)
BBa_M36723BBa_M36723 Version 1 (Component)mmoC (methane monooxygenase subunit C)
BBa_M36725BBa_M36725 Version 1 (Component)short-chain alkane hydroxylating device (mmoXYBZDC)
aspABBa_C0083 Version 1 (Component)aspartate ammonia-lyase
BBa_K511101BBa_K511101 Version 1 (Component)EYFP-FF4x4 MammoBlock
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.