Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:29
CI(1) micRNA asRNA
| Types | DnaRegion |
| Roles | mature_transcript_region RNA |
| Sequences | BBa_I1012_sequence (Version 1) |
Description
Region which serves as basis for transcription of asRNA that binds to and inhibitsNotes
References (unparsed) here:Mizuno, T., et al. Proc. Natl. Acad. Sci. USA (1984) 81, 1966-1970.
Coleman, J., et al. Cell (1984) 37, 429-36.
References (unparsed) here:
Mizuno, T., et al. Proc. Natl. Acad. Sci. USA (1984) 81, 1966-1970.
Coleman, J., et al. Cell (1984) 37, 429-36.
Complementary to beginning of
Anti-senseThe success of this system clearly rests on the ability to effectively and specifically target mRNA transcripts for degradation using anti-sense RNA. While many papers, articles, and books have been written on the subject, there are no consensus anti-sense building strategies presented. We thus chose to implement three different types of antisense inhibition: KISS, micRNA, and IS10. In the description that follows, the following nomenclature will be used:
target- the mRNA transcript that we wish to inhibit.
anti-sense- the anti-sense molecule which will bind and inhibit target.
micRNA
This anti-sense mechanism relies on two stem loops flanking an anti-sense sequence that is specific for the target. The function of the stem loops is to maintain the anti-sense region in a quasi-linear state. BBa_I1012 is built in this manner, with a linear region that will bind over the RBS, start codon, and 76 bp of BBa_I1010.
Incompatible with systems containing
Compatible with
Source
Custom design.| Sequence Annotation | Location | Component / Role(s) |
| stem_loop stem_loop Reverse Complement to cI mRNA Reverse Complement RBS reverse complement to cI cds start BBa_I1012 | 1,57 161,195 58,160 138,143 58,133 131,133 1,195 | stem_loop feature/stem_loop stem_loop feature/stem_loop feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature start_codon feature/start feature/BioBrick engineered_region |