Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:30
Custom cI(2) Protein w/LacO-1 Promoter (Used for Anti-sense inhibition)
| Types | DnaRegion |
| Roles | mature_transcript_region RNA |
| Sequences | BBa_I1060_sequence (Version 1) |
Description
Coding sequence for modified cI protein with LacO-1 promoter for antisense IS10 mRNA regulation.Notes
Can be used with all parts of the BBa_I system. It is inhibited by BBa_I1063. Incompatible with systems containing cI (wild type)
Source
Modified from BioBricks| Sequence Annotation | Location | Component / Role(s) |
| stem_loop rbs start cI lambda -35 -10 Complemenatry to Antisense mRNA I1063 SsrA 2 LacO-1 region LacO-1 start HH117a swap C added Binds BBa_I1063 swap swap swap swap BBa_I1060 | 56,85 73,78 83,85 83,835 21,26 44,49 56,158 797,835 830,835 1,20 27,43 56,56 58,58 86,88 89,90 72,72 79,79 81,81 82,82 1,835 | feature/stem_loop stem_loop ribosome_entry_site feature/rbs feature/start start_codon CDS feature/cds feature/promoter promoter promoter feature/promoter sequence_feature feature/misc feature/tag tag stop_codon feature/stop feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/start start_codon sequence_feature feature/misc sequence_alteration feature/mutation feature/binding non_covalent_binding_site sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature engineered_region feature/BioBrick |