Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: sarah hollingshead
Date created: 2007-10-21 11:00:00
Date modified: 2015-08-31 04:08:03
Zeaxanthin (yellow pigment) synthesis operon without PstI sites
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_I742139_sequence (Version 1) |
Description
Zeaaxnthin, a yellow pigment, is synthesised from farnesyl disphosphate in five sequential enzymatic steps.These five enzymes are encoded by the genes CrtE, CrtB, CrtI, CrtY and CrtZ.
More information on the zeaxanthin biosynthesis pathway may be found on the Edinburgh Yoghurt Wiki
Notes
Two PstI sites have been removed from CrtI gene via point mutation.All five genes were cloned so they retain their native ribosome binding sites.
Source
All five genes were isolated from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321)More information about carotenoid synthesis may be found in these references:
Diversity of Carotenoid Synthesis Gene Clusters from Environmental Enterobacteriaceae Strains, N Sedkova, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(12): 8141???8146 (2005)
Elucidation of Pathway the Erwinia uredovora Carotenoid Biosynthetic by Functional Analysis of Gene Products Expressed in Escherichia coli, N Misawa, et al, JOURNAL OF BACTERIOLOGY, 172(12): 6704-6712 (1990)
| Access | Instance | Definition |
| public public public public | BBa_I742116 BBa_I742133 BBa_I742117 BBa_I742118 | BBa_I742116 BBa_I742133 BBa_I742117 BBa_I742118 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I742116 BBa_I742133 BBa_I742117 BBa_I742118 | 1,954 963,3392 3401,4571 4580,5126 | BBa_I742116 BBa_I742133 BBa_I742117 BBa_I742118 |