BBa_I751501

BBa_I751501 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I751501

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kenichiro Iwasaki

Date created: 2007-10-24 11:00:00

Date modified: 2015-08-31 04:08:06

plux-cI hybrid promoter

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_I751501_sequence (Version 1)

Description

sense TWO INPUTS, activation by AHL and repression by LacI.

Notes

Based on the AHL regulated BioBrick part R0062 and the previous work on LacI regulated promoter (ref. 1), we designed a new promoter regulated by AHL and LacI. Since this promoter is a kind of a "HYBRID" of the two, we call it HYBRID PROMOTER.


Compared with existing BioBrick hybrid promoter R0065, this promoter is different in that its sequence is much shorter with lux box and -35 region overlapping, and that it is repressed by LacI, not cI.
Also, the output is clearly detected as shown in Fig. 3.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
OR1	48,66	non_covalent_binding_site, feature/binding
plux-cI hybrid promoter	19,66	promoter, feature/promoter
-35	19,25	sequence_feature, feature/misc
-10	42,47	sequence_feature, feature/misc
OR2	26,41	feature/binding, non_covalent_binding_site
luxR binding	1,18	non_covalent_binding_site, feature/binding

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I751501/1

Member of these Collections

• //chassis/prokaryote/ecoli
• //direction/forward
• //function/cellsignalling/luxr
• //promoter
• //regulation/multiple
• //regulation/negative
• //regulation/positive
• //rnap/prokaryote/ecoli/sigma70
• iGEM Parts Registry