Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Owen Koucky
Date created: 2016-07-09 11:00:00
Date modified: 2016-07-10 05:03:27
J100265 (pJC173b) with GFP replacing LuxAB
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_J100287_sequence (Version 1) |
Description
In their 2014 paper "Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., designed part J100265 to be used as an accessory plasmid in phage-assisted continuous evolution (PACE). This DNA part comes from a collection of 8 plasmids numbers J100264 - J100271. PACE employs M13 phage and E. coli in a chemostat. In order to conduct a PACE experiment of our own we used PCR and GGA to replace the LuxAB gene on part J100265 with the GFP gene to construct part J100287.Notes
We sought to replace LuxAB with GFP because we have greater familiarity with GFP fluorescence than we do with LuxAB luminescence.Source
"Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., 2014 with modifications made by Hartlee Johnston in A. Malcolm Campbell's lab at Davidson College.| Sequence Annotation | Location | Component / Role(s) |
| SC101 Origin of Replication T7 Promoter T7 Primer Bind SD8 RBS geneIII GFP bla (AmpR) AmpR bla Promoter RepA | 955,1700 1784,1803 1784,1803 1825,1839 1840,3113 3114,3830 3910,4770 3913,4572 4771,4965 1,951 | feature/misc sequence_feature promoter feature/promoter feature/primer_binding primer_binding_site feature/rbs ribosome_entry_site CDS feature/cds feature/cds CDS feature/cds CDS feature/cds CDS feature/promoter promoter CDS feature/cds |