BBa_J100304

BBa_J100304 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J100304
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: India Little
Date created: 2016-09-07 11:00:00
Date modified: 2016-09-08 01:50:23

NPT-II



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_J100304_sequence (Version 1)

Description

This promoter is constitutive. The -10 region is located at the 52nd base in the promoter, and the -35 region is located at the 27th base. The promoter has the ability to be cut by the EcoRI restriction enzyme.

Notes

The promoter had to be manipulated to fewer than 60 base pairs.

Source

The promoter was found in 'Letters in Applied Microbiology' titled 'AiiA-mediated quorum quenching does not affect virulence or toxoflavin expression in Burkholderia glumae SL2376'. The NPT-II promoter is originally short, with fewer than 80 bases in its entirety. The part of the promoter has been trimmed to within twelve bases of the -10 and -35 regions.

Sequence Annotation Location Component / Role(s)
-10 box
-35 box
36,42
14,20
feature/binding non_covalent_binding_site
feature/binding non_covalent_binding_site
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J100304/1