TTSv3

BBa_J11022 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J11022
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Emanuel Nazareth
Date created: 2005-10-04 11:00:00
Date modified: 2015-08-31 04:08:25

Tunable Temperature Sensor v3 (Registry 7.05 DNA-1 + Harvard Parts)



Types
DnaRegion

Roles
engineered_region

Project

Sequences BBa_J11022_sequence (Version 1)

Description

Tuneable Temperature Sensor made with parts available in Registry 7.05 DNA-1. At higher temperatures, the lacI dimer degrades into lacI monomers. The LacI promoter is no longer inhibited, hence more RFP is produced. Also, cI 434 is produced resulting in Prm+ being repressed, thus lowering the levels of GFP. At lower temperatures, the lacI dimer is allowed to form, hence the LacI promoter is repressed. RFP levels decrease, and cI levels drop. This results in Prm+ no longer being repressed, hence GFP levels increase. Behaviour is tuneable by adjusting the levels of lacI ts via L-arabinose activating pBad. The net result is that the fluorescence (green to red) can be correlated to a temperature (at a given level of L-arabinose).

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J11022/1