| Types | DnaRegion
|
| Roles | promoter
Regulatory
|
| Sequences | BBa_J31014_sequence (Version 1)
|
Description
short sequence that is inserted in front of a coding region without an RBS as the part includes an RBS. When in front of the sequence and no taRNA is present, the transcript will not be translated.
Notes
The Xba/SpeI scar became the spacer between the coding and RBS; therefore, the first few bases of the crRNA were changed to the reverse complement of the scar. Currently needs to be grown up in dam/- cells in order to cut with Xba.
Source
Generated by primer dimer PCR (see Erin Zwack's OWW page for the protocol). The original sequence is a slightly modified version of Isaacs et al.'s riboregulator crRNA.
