Wintergree

BBa_J45181 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J45181

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Stephen Payne

Date created: 2007-02-10 12:00:00

Date modified: 2015-08-31 04:08:49

Exponential phase dependent wintergreen odor generator

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_J45181_sequence (Version 1)

Description

This part converts salicylic acid into methyl salicylate (a mint-smelling compound) in the exponential phase of cell growth. The part consists of a stationary phase promoter (J45992), connected to an inverter (Q04401) as well as the methyl salicylate protein generator (J45119). This part replaces the defective part J45180, which used the truncated version of the stationary phase promoter (J45993). This part did not function as it was designed due to an imbalance between the strength of the stationary phase promoter and the strength of the promoter in the inverter (R0040).

Notes

We needed the full osmY promoter strength to be relatively comparable to the R0040 promoter strength.

Source

Yes. It comes from many subparts.

Components

Access	Instance	Definition
public	BBa_J45994	Exponentia
public	BBa_J45119	BSMT1

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J45994	1,1109	Exponentia
BBa_J45119	1118,2347	BSMT1

Other Properties

• igem#experience: Issues
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J45181/1

Member of these Collections

• //function/odor
• //s
• iGEM Parts Registry