p(osmY)

BBa_J45993 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J45993
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stephen Payne
Date created: 2006-07-10 11:00:00
Date modified: 2015-08-31 04:08:50

Minimal stationary phase osmY promoter



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_J45993_sequence (Version 1)

Description

osmY's promoter is active in stationary phase and under high osmotic pressure conditions.

Notes

Truncated Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GGCT TAT GTT TTC GCT GAT ATC - 3'

Total Length: 49 bp

Annealing Length: 21 bp

GC Content: 38.1%

Melting Temperature: 49.5 degrees C

hairpin deltaG: -2.66 kcal/mol

self dimer deltaG: -99.97 kcal/mol

Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3'

Total Length: 54 bp

Annealing Length: 25 bp

GC Content: 20.0%

Melting Temperature: 46.4 degrees C

hairpin deltaG: -2.86 kcal/mol

self dimer deltaG: -99.44 kcal/mol

heterodimer deltaG with Conserved Forward Primer: -99.97 kcal/mol

Source

This part was PCRed out of E. coli's genome.

Sequence Annotation Location Component / Role(s)
-10
-35
46,51
22,27
promoter feature/promoter
promoter feature/promoter
igem#experience
Fails
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J45993/1