Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dan Widmaier
Date created: 2008-01-16 12:00:00
Date modified: 2015-08-31 01:56:26
pCASP SPI-1 Secretion Circuit
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_J64032_sequence (Version 1) |
Description
The pCASP circuit is a protein expression and secretion circuit. The system is currently only functional when expressed in Salmonella under SPI-1 expressing conditions. When SPI-1 is expressed the pCASP circuit will be activated for expression at the same time natural effectors would normally turn on and secrete. This circuit will express a protein of interest fused to the SptP167 secretion signal along with co-expression of the sicP chaperone. This combination is sufficient to direct the protein of interest to the export machinery and elicit secretion to the extracellular environment. In the initial configuration of this circuit the DH guanine exchange factor is used as a de-bugging secreted protein.Note: Some evidence exists of impassible substrates for the secretion machinery. Most of these proteins are highly thermodynamically stable domains that cannot be unfolded to transit through the secretion system.
Notes
This device was not designed with biobrick standard assembly in mind and has several of the scar sequence sites throughout the circuit.Source
This is a composite part consisting of both natural and synthetic DNA components| Access | Instance | Definition |
| public public public public public public public | BBa_J64001 BBa_J64022 BBa_J64008 BBa_J64007 BBa_J64004 BBa_J64005 BBa_J64043 | BBa_J64001 BBa_J64022 SicP-SptP BBa_J64007 BBa_J64004 3x FLAG BBa_J64043 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J64001 BBa_J64022 BBa_J64008 BBa_J64007 BBa_J64004 BBa_J64005 BBa_J64043 | 1,143 144,163 164,1043 1044,1070 1071,1691 1692,1772 1773,1877 | BBa_J64001 BBa_J64022 SicP-SptP BBa_J64007 BBa_J64004 3x FLAG BBa_J64043 |