Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jeffrey J Tabor
Date created: 2007-12-12 12:00:00
Date modified: 2015-05-08 01:08:17
LuxR+3OC6HSL independent R0065
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_J64067_sequence (Version 1) |
Description
The -35 box of R0065 has been modified to promote constitutive (LuxR+3OC6HSL-independent) gene expression. An extended -10 sequence has also been added to increase absolute transcription. The promoter still contains the operators OR1 and OR2 such that it retains its sensitivity to CI.Notes
see aboveSource
modified from R0065| Sequence Annotation | Location | Component / Role(s) |
| -35 extended -10 -10 lux box +1 OR2 OR1 | 25,30 43,46 48,53 6,26 59,59 58,75 82,98 | promoter feature/promoter promoter feature/promoter promoter feature/promoter feature/operator operator feature/misc sequence_feature operator feature/operator feature/misc sequence_feature |