BBa_J64800

BBa_J64800 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J64800
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: laura lavery
Date created: 2007-03-24 12:00:00
Date modified: 2015-05-08 01:08:17

RHLR/RHLI Inducible & LasR/LasI repressible Promoter



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_J64800_sequence (Version 1)

Description

This part is similar to BBa_R0079 but has an added binding site for RHLR/RHLI in between the -35 and -10 region. Thus this promoter is induced by LasR/LasI but repressed by RHLR/RHLi binding.

Notes

There is a 17bp spacer between the -35 and -10 regions and the binding site for RHLR/RHLI is 20bp long. I was able to fit the whole binding site in the spacer region because the last three base pairs of the -35 region and the first three base pairs of the RHLR/RHLI binding site are the same.

Source

Rust L. et. al., "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Journal of Bacteriology, 1996.

Sequence Annotation Location Component / Role(s)
RHLR/RHLI
LasR/LasI
-35
-10
1,23
23,40
19,24
42,47
feature/binding non_covalent_binding_site
operator feature/operator
sequence_feature feature/misc
sequence_feature feature/misc
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J64800/1